At least 150 Palestinians have been injured in clashes with Israeli police at the al-Aqsa Mosque compound in Jerusalem, Palestinian officials said.
Israeli police said officers entered the site after coming under attack with fireworks, stones and other objects.
Three Israeli police were hurt, they said.
The flashpoint site is deeply important to Muslims and Jews, who know it as the Temple Mount, and is at the heart of competing historical claims.
Israeli-Palestinian tensions have soared recently after deadly attacks in occupied West Bank.
A 17-year-old Palestinian wounded on Thursday during an Israeli operation in the area of Jenin in the northern West Bank died on Friday, the Palestinian health ministry said.
More than 20 Palestinians have been killed since Israel stepped up sweeps in the West Bank, centring on Jenin district after the killings of three Israelis in Tel Aviv last Thursday.
The Palestinian foreign ministry condemned Israel’s actions at the compound, saying it held it “fully and directly responsible for this crime and its consequences”.
The clashes come after militant groups in Gaza called for “hundreds of thousands” of Palestinians to converge on the compound on Friday “to protect our nation and our mosque”.
Last year Hamas, which governs Gaza, fired rockets towards Jerusalem following clashes at the mosque compound after weeks of unrest in the city, triggering a devastating 11-day war with Israel.
The compound is known to Muslims as Haram al-Sharif and is also considered, in its entirety, as al-Aqsa Mosque. It is the third holiest site in Islam.
The same ground is sacred to Jews as the location of two biblical temples, and is the holiest site in Judaism.
The recent surge in violence comes at a particularly volatile time, with the start of the Jewish festival of Passover on Friday night coinciding with the Islamic holy month of Ramadan and the Christian festival of Easter.